Product fluorescent-dyes-nir-ii

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Catalog	name	Description	price
R-M-5455	IR 650 DBCO	IR 650 DBCO (IRDye® 650 DBCO analog) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.IR 650 DBCO routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. IR 650 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.	price>
R-M-5456	IR 680LT Maleimide	IR 680 (IRDye® 680 equivalent) is a bright, water-soluble dye that is structurally very similar to the commonly used Cy®5.5 Dye. The water solubility, brightness, and self-quenching characteristics of this dye were significantly improved by the introduction of two additional sulfo groups. Far-red fluorescent IR 680 conjugates are spectrally similar to other 680 nm dyes that are used for flow cytometry, microscopy and other applications. Proteins can be labeled with IR 680 at high molar ratios without significant self - quenching, leading to brighter conjugates and more sensitive detection.Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.	price>
R-M-5457	IR 680LT NHS ester	IR 680LT (IRDye® 680LT equivalent) is a bright, water-soluble dye that is structurally very similar to the commonly used Cy®5.5 Dye. The water solubility, brightness, and self-quenching characteristics of this dye were significantly improved by the introduction of two additional sulfo groups. Far-red fluorescent IR 680 conjugates are spectrally similar to other 680 nm dyes that are used for flow cytometry, microscopy and other applications. Proteins can be labeled with IR 680 at high molar ratios without significant self - quenching, leading to brighter conjugates and more sensitive detection.The NHS ester (succinimidyl ester) is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules.	price>
R-M-5458	IR 750 NHS ester	IR 750 NHS Ester (IRDye® 750 NHS Ester analog) is a water-soluble, bright, near-IR fluorescent dye. IR 750 conjugates ideally suited for the 633 nm laser lines or dye-pumped laser. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with IR 750 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. IR 750 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.	price>
R-M-5459	IR 750 Maleimide	IR 750 Maleimide (IRDye® 750 Maleimide analog) is a water-soluble, bright, near-IR fluorescent dye. IR 750 conjugates ideally suited for the 633 nm laser lines or dye-pumped laser. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. IR 750 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.	price>
R-M-5460	IR 750 Azide	IR 750 Azide (IRDye® 750 Azide analog) is a water-soluble, bright, near-IR fluorescent probe that is pH-insensitive from pH 4 to pH 10. Its excitation band is ideally suited for the 633 nm laser lines or dye-pumped laser. IR 750 dye is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.IR 750 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.	price>
R-M-5461	IR 750 Picolyl Azide	IR 750 Picolyl Azide (IRDye® 750 Picolyl Azide analog) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper.In addition, the use of IR 750 Picolyl Azide instead of conventional IR 750 Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.	price>
R-M-5462	IR 750 Alkyne	IR 750 Alkyne (IRDye® 750 Alkyne analog) is a water-soluble, bright, near-IR fluorescent probe that is pH-insensitive from pH 4 to pH 10. Its excitation band is ideally suited for the 633 nm laser lines or dye-pumped laser. IR 750 dye is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.	price>
R-M-5463	IR 750 DBCO	IR 750 DBCO (IRDye® 750 DBCO analog) is a water-soluble, bright, near-IR fluorescent probe that is pH-insensitive from pH 4 to pH 10. Its excitation band is ideally suited for the 633 nm laser lines or dye-pumped laser. IR 750 DBCO is mainly used for imaging of azide-containing biomolecules without the need for copper catalyst. It reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In applications like staining the surface of live cells or if there are concerns about native protein function loss in the presence of copper IR 750 DBCO is an excellent alternative to copper requiring fluorescent alkynes.IR 750 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.	price>
R-M-5468	MB 543 NHS ester	MB™543 is a very hydrophilic, water-soluble orange fluorescent dye.Characteristic features of MB™543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.	price>

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