Product fluorescent-dyes-MB-dyes

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Catalog	name	Description	price
R-M-5464	MB 488 NHS ester	MB™488 is a very hydrophilic, water-soluble green emitting dye.Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™488 is an excellent dye for flow cytometry (FACS),fluorescence in-situ hybridization (FISH) and many other applications.The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.	price>
R-M-5465	MB 488 Maleimide	MB™488 is a very hydrophilic, water-soluble green emitting dye.Characteristic features of the label are strong absorption, high fluorescence quantum yield,and very high photostability. MB™488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™488 is an excellent dye for flow cytometry(FACS),fluorescence in-situ hybridization (FISH) and many other applications.Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups,–SH) at pH 6.5 to 7.5 to form stable thioether bond.The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.	price>
R-M-5466	MB 488 Picolyl Azide	MB™488 is a very hydrophilic, water-soluble green emitting dye.Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™488 is an excellent dye for flow cytometry (FACS),fluorescence in-situ hybridization (FISH) and many other applications.MB™488 Picolyl Azide is an advanced probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper.	price>
R-M-5467	MB 488 Alkyne	MB™488 is a very hydrophilic, water-soluble green emitting dye.Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability.MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM,dSTORM, STED etc. Additionally MB™488 is an excellent dye for flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many other applications.MB 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (MB™488 DBCO) is also available for application where the presence of copper is not acceptable.	price>
R-M-5471	MB 543 Alkyne	MB™543 is a very hydrophilic, water-soluble orange fluorescent dye. Structurally related to Alexa Fluor® 546 dye, MB™543 dye contains an additional, negatively charged sulfo group (SO3H) to improve water solubility and minimize self-quenching. Characteristic features of MB™543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.MB™543 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (MB™543 DBCO) is also available for application where the presence of copper is not acceptable.	price>
R-M-5472	MB 543 DBCO	MB™ 543 is a very hydrophilic, water-soluble orange fluorescent dye. Characteristic features of MB™ 543 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.MB™ 543 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB™ 543 DBCO is an ideal alternative to copper requiring fluorescent alkynes.	price>
R-M-5473	MB 594 NHS Ester	MB 594 NHS Ester is a very hydrophilic, water-soluble red fluorescent dye. Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.	price>
R-M-5474	MB 594 Azide	MB™ 594 is a very hydrophilic, water-soluble red fluorescent dye.Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.MB 594 Azide reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.	price>
R-M-5475	MB 594 Picolyl Azide	MB™ 594 is a very hydrophilic, water-soluble red fluorescent dye.Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.MB 594 Picolyl Azide is an advanced probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper.In addition, the use of picolyl azide instead of conventional azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.	price>
R-M-5476	MB 594 Alkyne	MB™594 is a very hydrophilic, water-soluble red fluorescent dye.Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™ 594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc.MB 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less detection (MB™ 594 DBCO) is also available for application where the presence of copper is not acceptable.	price>

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