Recombinant Strep-Tactin with Cysteine

Streptavidin is a streptavidin mutant, which can bind specifically to streptavidin. The Strep II tag is a small 8 amino acid tag (wshpqfek). Since the tag is small, it is only about 1 kDa, which generally does not affect the structure and function of the fusion protein. It is commonly used in the detection and purification of fusion protein. Compared to streptactin, streptactin has a 10 times stronger affinity for streptavidin. It can bind and dissociate with streptavidin under mild conditions and can play a good role in separation and purification. In order to be able to bind to the medium, a cysteine ​​has been added to the C-terminus of the amino acid sequence, which can be covalently linked to the medium through a sulfhydryl group.

msds of Recombinant Strep-Tactin with Cysteine