MB 594 Maleimide

MB™594 is a very hydrophilic, water-soluble red fluorescent dye.Characteristic features of MB™ 594 are strong absorption, high fluorescence quantum yield, high photostability, and pH insensitivity from pH 3 to pH 10. MB™594 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM,STED etc.Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups,–SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

msds of MB 594 Maleimide