D4476 CAS:301836-43-1

D4476 is a relatively specific cell osmotic inhibitor of CK1 and ALK5 with IC50 values of 0.3 and 0.5,respectively μ In vitro,the phosphorylation of CK1 (casein kinase 1)was located at the serine residue near another phosphoserine in the multipoint phosphorylation domain(MPD).D4476 may be a competitive ATP inhibitor of CK1.In H4IIE cells,the site-specific phosphorylation of FOXO1a (ser322 and ser325)was inhibited at 50 and 125 μ M.The initial rate of nuclear repulsion is reduced.In HeLa cells,d4476 inhibited CK1 induced RhoB phosphorylation(ser185)at 50℃μ RhoB dependent actin fiber formation is then activated.D4476 treaCAS No.285986-88-1t resulted in partial p53 dependent growth arrest of HCT116 cells.

msds---D4476