Tf-PEG-CDDP

Objective to prepare transferrin (TF) modified cisplatin long circulating liposomes (TF peg CDDP) and investigate its physical properties and in vitro release characteristics.Methods blank liposomes were prepared by membrane dispersion ultrasonic method,and loaded with cisplatin by ammonium sulfate gradient method.TF peg CDDP was prepared by linking transferrin with terminal active group of dspe-peg2000-mal;The encapsulation efficiency of TF peg CDDP was determined by UV VIS spectrophotometer.The particle size was determined by laser scattering particle size analyzer and its properties were observed by transmission electron microscope.The sustained release ability of cisplatin liposomes in vitro was detected by dialysis method.Results TF peg CDDP was round vesicle like structure, slightly uneven in size,and the average particle size was 200 nm,The average entrapment efficiency was (78.222±5.4086)%,and the stability was acceptable.The in vitro release test showed that TF peg CDDP had better sustained-release ability than CDDP.Conclusion TF peg CDDP has sustained-release ability and is an effective cisplatin targeted preparation.

msds---Tf-PEG-CDDP