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Home > products > synthetic-chemistry > protein-crosslinkers > rb-ek

Recombinant Bovine(rb-EK)

Recombinant Bovine-Enterokinase

Catalog # Pkg Size Price(USD) Quantity Buy this product
R-L-156 500U 459.00
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R-L-156 1000U 709.00
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Product description

Enterokinase is a serine proteolytic enzyme, which can recognize the sequence of ASP ASP Lys (ddddk) in the protein efficiently and specifically, hydrolyze the peptide bond at the C-terminal of lysine (Lys , K), produce cleavage, hydrolyze trypsinogen to trypsin in the organism. Because enterokinase has high specificity and efficient enzymatic cleavage, it is widely used in genetic engineering.

Activity definition: 25 ℃, reaction buffer 50 mM Tris-HCl (pH 8.0), substrate 50 μ G 1 mg/ml of fusion protein containing restriction site, the amount of enzyme required to cut 95% of fusion protein within 16 hours is 1 enzyme activity unit (1 U).


Appearance Liquid
Molecular Weight N/A
Purity >95% as determined by SDS-PAGE
Solubility N/A
Synonym Recombinant Bovine Enterokinase(rb-EK)
Buffer 50 mM Tris-HCl, 50%Glycerol, pH 8.0
Tag C-terminal His Tag
Enzyme digestion reaction conditions 25 ℃, enzyme digestion for 16 hours
Specific activity >30 KU/mg, which is 1mg of recombinant bovine enterokinase, can cleave at least 1.5g of fusion protein
Storage -20℃, protected from light and moisture
Transportation 4-25℃ temperature for up to 2 weeks
Stability 1 year
Document

msds of Recombinant Bovine
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