poly(β-amino esters) Encapsulated DNA

The main synthesis method of PBAE is Michael addition reaction.The results showed that PBAE with different end groups had different gene transfection efficiency,and the hydrophilic amino group containing hydroxyl or extra amine had the best transfection efficiency;the amino group in the polymer was protonated under acidic conditions,and could bind to the drug or DNA containing negative ions,so that it could be effectively encapsulated.Because there were only tertiary amines in the straight chain PBAE,and there might be primary and secondary amines in the branched chain PBAE,When DNA nanoparticles were formed, the encapsulation ability of branched chain PBAE was stronger than that of straight chain PBAE,and the diameter of branched chain amino ester nanoparticles was smaller.

msds---poly(β-amino esters) Encapsulated DNA