To verify the potential of targeting distribution of peptide guided liposome drug delivery system,the peptide can be coupled to the polyethylene glycol end of DSPE-PEG through the specific reaction between the sparse group and the maleimide group through the linker SPDP,and the linked products were analyzed by HPLC. We used post insertion technique to combine ph1-peg-dspe micelles with liposomes（ HSPC:Chol Methods:DSPE-PEG=2:1:0.1) were co incubated to prepare targeted liposomes.However,the expression of phe2 in tumor cells was higher by Lipofectamine conjugation and liposome conjugation.Ph1 coupled with cisplatin loaded long circulating liposomes can greatly improve the cytotoxicity of cisplatin liposomes to Tie2 positive cells.

msds----PH1-PEG750-DSPE