X
Email:
sales@ruixibiotech.com

HCV Protease FRET Substrate (RET S1)

HCV Protease FRET Substrate (RET S1)

Catalog # Pkg Size Price(USD) Quantity Buy this product
R-M-1605 1mg 590.00
- +
+ Add to cart

Product description

A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.


Appearance N/A
Molecular weight N/A
Purity >90%
Solubility N/A
Sequence Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 (trifluoroacetate salt)/ Ac-DED(EDANS)-EE-Abu-psi-(COO)AS-K(DABCYL)-NH2
Molecular Formula C68H89N15O25S1
Storage -20℃, protected from light and moisture
Transportation 4-25℃ temperature for up to 3 weeks
Stability 1 year
Document

Related Product