7-AMCA maleimide

7-amca dye is a very common dye. It is a coumarin dye excited by UV and emitting blue fluorescence (ex/em ~ 353/442). It can be used in combination with the fluorescence of various other colors. Like other blue fluorescent dyes, AMCA fluorescence is not very bright and is only suitable for target dyeing with high abundance.Maleimide is a group commonly used in biomarker reaction. It can generate stable thioether structure through affinity addition reaction with sulfhydryl (- SH), so as to realize labeling. Although amino groups (such as lysine arginine side chain) also have affinity, maleimide selectively labels sulfhydryl groups in neutral or slightly acidic buffer (reaction speed > 1000 times faster than amino groups). The labeling reaction has the advantages of rapid reaction, high selectivity and good yield. In addition, sulfhydryl is a functional group widely existing in biomolecules, such as serine and disulfide bond in proteins. Therefore, maleimide / thiol labeling has become the most commonly used biological ligation reaction second only to NHS / amino labeling.

msds of 7-AMCA maleimide