5-TAMRA azide
5-Carboxytetramethylrhodamine azide
5-TAMRA azide,5-Carboxytetramethylrhodamine azide from ruixi.5-TAMRA Azide (also known as TAMRA azide,isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc,tungsten and xenon arc lamps,the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantlyused for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction(CUAAC). It also reacts with strained cyclooctyne via a copper-free“click chemistry”reactionto form a stable triazole and does not require Cu-catalyst or elevated temperatures.Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dyefor staining proteins,labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotideslabeled with a single isomer TAMRA usually give better resolution in HPLC purification that isoften required in the conjugation processes.
Appearance | N/A |
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Molecular weight | N/A |
CAS | 825651-66-9 |
PDI by GPC | <1.5 |
Solubility | Organic solvents |
Storage | -20℃, protected from light and moisture |
Transportation | 4-25℃ temperature for up to 2 weeks |
Stability | 1 year |