5(6)-TAMRA-maleimide

5(6)-TAMRA-maleimide,5(6)-Tetramethylrhodamine-maleimide from ruixi.Tetramethylrhodamine,namely tetramethylrhodamine (TMR, Tamra), is a fluorescent dye commonly used in biological coupling. It is often used to connect antibodies, avidin and so on for immunochemistry. TMR and Tamra are the common names of this dye in the literature. Tamra generally introduces carboxylic acid at position 5 or 6 for labeling. Maleimide is a group commonly used in biomarker reaction. It can generate stable thioether structure through affinity addition reaction with sulfhydryl (- SH), so as to realize labeling. Although amino groups (such as lysine arginine side chain) also have affinity, maleimide selectively labels sulfhydryl groups in neutral or slightly acidic buffer (reaction speed > 1000 times faster than amino groups). The labeling reaction has the advantages of rapid reaction, high selectivity and good yield. In addition, sulfhydryl is a functional group widely existing in biomolecules, such as serine and disulfide bond in proteins. Therefore, maleimide / thiol labeling has become the most commonly used biological ligation reaction second only to NHS / amino labeling.

msds of 5(6)-TAMRA-maleimide